Published in
Elsevier, Biomolecular Engineering, 3(17), p. 83-85, 2001
DOI: 10.1016/s1389-0344(00)00070-8
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Enhanced green fluorescent protein as a useful tag for rapid identification of homozygous transgenic mice
Journal article published in 2001 by
Masahiro Sato,
Toshiteru Watanabe,
Akiko Oshida,
Ayako Nagashima,
Jun-Ichi Miyazaki 
,
Minoru Kimura
,
Minoru Kimura
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Abstract
We developed a technique that simplifies the process of confirming homozygous transgenics at preimplantation stages, which are the earliest stages used in test breeding, using enhanced green fluorescent protein as a tag. All the blastocysts obtained by mating with the combination of Tg/Tg male (homozygous for transgene) x +/+ female exhibited fluorescence.