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Public Library of Science, PLoS ONE, 9(9), p. e107425, 2014

DOI: 10.1371/journal.pone.0107425

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Using Amino-Labeled Nucleotide Probes for Simultaneous Single Molecule RNA-DNA FISH

Journal article published in 2014 by Reelina Basu, Lan-Tian Lai, Zhenyu Meng, Jun Wu, Fangwei Shao, Li-Feng Zhang ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long non-coding RNAs being identified in the current research. With probes either chemically or biologically synthesized, we demonstrate that the method can be applied to study a wide range of RNA and DNA targets at the single-cell and single-molecule level in cellular contexts.