Two ectomycorrhizal macrofungal Amanita species of the section Lepidella, A. strobiliformis and A. solitaria, were found to hyperaccumulate silver (Ag). All samples were collected from non-argentiferous areas with background Ag content in soils (0.07-1.01 mgkg(-1) Ag). The Ag contents of both Amanita species were mostly in the range of 200-700 mgkg(-1)D.W. with the highest Ag content of 1253 mgkg(-1) in one sample of A. strobiliformis. Silver concentrations in macrofungal fruit bodies were commonly 800-2500 times higher than in underlying soils. A. strobiliformis and A. solitaria are the first eukaryotic organisms known to hyperaccumulate Ag.