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Humana Press, Methods in Molecular Biology, p. 151-174, 2010

DOI: 10.1007/978-1-61737-967-3_9

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Semi-synthesis of Glycoproteins from E. coliThrough Native Chemical Ligation

Book chapter published in 2010 by Jonathan P. Richardson ORCID, Derek Macmillan
This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

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Abstract

Sufficient quantities of homogeneous samples of post-translationally modified proteins are often not readily available from biological sources to facilitate structure-function investigations. Native chemical ligation (NCL) is a convenient method for the production of biologically active proteins from smaller fragments. Such an approach allows protein modifications to be introduced in a controlled fashion into smaller peptide fragments which are amenable to total chemical synthesis. These fragments of defined sequence and structure can be elaborated to full-length proteins through NCL reactions with suitable components derived from bacterial origin. This report describes methods for the bacterial production of components for NCL and their use in typical reactions.