Infestation of flies of the genus Megistopoda (Diptera, Strebilidae) on bats (Mammalia, Chiroptera) in an urban fragment of Cerrado in Campo grande, Mato Grosso do Sul. This study aimed to analyze the infestation of bats by flies of the genus Megistopoda in an urban forest remnant of Campo Grande, MS. The bats were captured with mist nets in monthly samples taken in two periods, the first between September 2011 and July 2012, and the second between October 2012 and May 2013, taking turns in 12 sampling points. 354 captures of bats belonging to 13 species were performed; yielding 27 samples of Megistopoda aranea species parasitizing three host species. The Diptera M. aranea was found mainly parasitizing the Artibeus planirostris species, occurring in 21 specimens, resulting in a prevalence of 29.57% of the population of this bat. For Artibeus lituratus specimens of M. aranea were found only five individuals, with a prevalence of 3.57%. A specimen of Carollia perspicillata parasitized by this Diptera, with a prevalence of 3.12% for this host species has been verified. The low prevalence of M. aranea in A. lituratus says little monospecific interaction between these parasites and hosts.