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Public Library of Science, PLoS ONE, 3(7), p. e34322, 2012

DOI: 10.1371/journal.pone.0034322

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Overexpression of DNA Polymerase Zeta Reduces the Mitochondrial Mutability Caused by Pathological Mutations in DNA Polymerase Gamma in Yeast

Journal article published in 2012 by Enrico Baruffini ORCID, Fausta Serafini, Iliana Ferrero, Tiziana Lodi
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

In yeast, DNA polymerase zeta (Rev3 and Rev7) and Rev1, involved in the error-prone translesion synthesis during replication of nuclear DNA, localize also in mitochondria. We show that overexpression of Rev3 reduced the mtDNA extended mutability caused by a subclass of pathological mutations in Mip1, the yeast mitochondrial DNA polymerase orthologous to human Pol gamma. This beneficial effect was synergistic with the effect achieved by increasing the dNTPs pools. Since overexpression of Rev3 is detrimental for nuclear DNA mutability, we constructed a mutant Rev3 isoform unable to migrate into the nucleus: its overexpression reduced mtDNA mutability without increasing the nuclear one.