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Humana Press, Methods in Molecular Biology, p. 71-85

DOI: 10.1007/978-1-4939-1619-1_7

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Identification of microRNAs in Human Plasma

Journal article published in 2014 by Bram C. van der Eerden ORCID, Rodrigo D. Alves, Christel E. Kockx ORCID, Zeliha Ozgur, Marijke Schreuders-Koedam, Jeroen van de Peppel, Wilfred F. van Ijcken ORCID, Johannes P. van Leeuwen ORCID
This paper is available in a repository.
This paper is available in a repository.

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Abstract

In recent years, microRNAs (miRNA) have been demonstrated to be present in body fluids and may therefore serve as diagnostic markers for diseases. By characterizing miRNA profiles in plasma, a miRNA signature may potentially be developed as a diagnostic and risk assessment tool for particular (patho)physiological states. This chapter describes the isolation, purification, identification, and sequencing of human plasma miRNAs.