Nursing restriction and parity might be stressful factors influencing the challenging post-partum period. This experiment aimed to evaluate the effects of nursing frequency (in multiparous dams) and parity on faecal glucocorticoid metabolites after calving in beef cows (n=64). Also, the role of these effects on productive, metabolic and reproductive functions was assessed. From the day after calving, forty-six Parda de Montaña cows were assigned at random to three nursing frequencies: once-daily nursing (1×30min at 0800h) (RESTR1), twice-daily nursing (2×30min at 0800 and 1530h) (RESTR2) and ad libitum nursing (ADLIBC). Eighteen heifers were maintained with free access to their calves as in the ADLIB cow's group (ADLIBH). Data were analysed through analysis of variance and survival analysis.The faecal glucocorticoid metabolites during the first three days post-partum were not affected by either calf management or parity (P>0.10) but they were greater at 12h post-partum than in subsequent samplings (P0.10), but their mean concentration was lower in ADLIBC than in ADLIBH (P