Cell‐Free Transcription/Translation from PCR‐Amplified DNA for High‐Throughput NMR Studies
Journal article published in 2007 by
Peter S. C. Wu,
Kiyoshi Ozawa,
Siew P. Lim,
Subhash G. Vasudevan,
Nicholas E. Dixon 
,
Gottfried Otting
,
Gottfried Otting
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Abstract
Quick and clean: Excellent protein yields in cell-free protein synthesis can be obtained by using polymerase chain reaction (PCR) amplified DNA templates, provided that the templates are designed for cyclization. This eliminates time-consuming cloning steps and allows one to go from complementary DNA to the protein NMR spectrum in 24 h. (Graph Presented).