In this study, a mixed culture specifically for thermophilic anaerobic treatment of Palm Oil Mill Effluent (POME) has been cultivated from a mesophilic seed sludge using a batch fermenter. The performance of the cultivated mixed culture on the treatment of POME was also evaluated with the batch fermenter. The cultivation was successful as the cultivated thermophilic mixed culture managed to reduce at least 90 % of Chemical Oxygen Demand (COD) in POME with after 6 days of operation from POME treatment with a Mixed Liquor Suspended Solid (MLSS) concentration of 14000 mg/L producing biogas with 66% of methane.