The assessment of regional vulnerability to climate change has become an important issue in climate change adaptation. In order to aid decision making in terms of prioritising adaptation action or allocating resources for adaptation measures, both scholars and policy makers emphasise the need for comprehensive and spatially explicit vulnerability studies. Adaptive capacity is not only an important part of vulnerability assessments, it also underlies and enables the governing of adaptation activities, thus making it an issue relevant to policy. Hence, the assessment of adaptive capacity gives decision makers on international, national and regional level important information to develop adaptation policy. Drawing on current vulnerability and adaptive capacity studies, the objective of this paper is to present a framework that structures adaptive capacity assessments based on science-policy interaction, discussing the objectives of the assessment, the methodology and the use of results. The framework is applied to two examples: a pan-European assessment of adaptive capacity and an assessment of the adaptive capacity of the tourism sector in the European Alps, both on the regional level. The main findings show how decisions related to methodology can influence the use of results in decision making. In conclusion, this paper contributes to the literature by arguing that more attention needs to be paid to assessment design in order to better support the mobilisation of adaptive capacity for adaptation.