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American Society for Microbiology, Journal of Clinical Microbiology, 9(50), p. 3093-3095, 2012

DOI: 10.1128/jcm.01045-12

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Formic Acid-Based Direct, On-Plate Testing of Yeast and Corynebacterium Species by Bruker Biotyper Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Journal article published in 2012 by Elitza S. Theel, Bryan H. Schmitt, Leslie Hall, Scott A. Cunningham ORCID, Robert C. Walchak, Robin Patel, Nancy L. Wengenack
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT An on-plate testing method using formic acid was evaluated on the Bruker Biotyper matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry system using 90 yeast and 78 Corynebacterium species isolates, and 95.6 and 81.1% of yeast and 96.1 and 92.3% of Corynebacterium isolates were correctly identified to the genus and species levels, respectively. The on-plate method using formic acid yielded identification percentages similar to those for the conventional but more laborious tube-based extraction.