Dissemin is shutting down on January 1st, 2025

Published in

American Society for Microbiology, Journal of Clinical Microbiology, 8(50), p. 2783-2785, 2012

DOI: 10.1128/jcm.00284-12

Links

Tools

Export citation

Search in Google Scholar

Evaluation of the New NucliSENS EasyQ KPC Test for Rapid Detection of Klebsiella pneumoniae Carbapenemase Genes ( bla <sub>KPC</sub> )

Journal article published in 2012 by Teresa Spanu, Barbara Fiori, Tiziana D'Inzeo, Giulia Canu, Serena Campoli, Tommaso Giani, Ivana Palucci ORCID, Mario Tumbarello, Maurizio Sanguinetti, Gian Maria Rossolini
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

Full text: Download

Green circle
Preprint: archiving allowed
Upload
Green circle
Postprint: archiving allowed
Upload
Orange circle
Published version: archiving restricted
Upload
Policy details
Data provided by SHERPA/RoMEO

Abstract

ABSTRACT KPC-type carbapenemases are emerging in Klebsiella pneumoniae and other Gram-negative pathogens worldwide. Rapid and sensitive detection of these resistance determinants has become relevant to clinical management and infection control. We evaluated the bioMérieux EasyQ real-time PCR assay for bla KPC detection with 300 members of the Enterobacteriaceae , including 29 control strains producing known carbapenemases and 271 nonreplicate clinical isolates. The EasyQ assay correctly detected all of the 111 isolates harboring bla KPC genes, with no false positives, and results were available within 2 h.