American Chemical Society, Journal of Organic Chemistry, 26(62), p. 9024-9030, 1997
DOI: 10.1021/jo970589t
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ODNs containing a 3‘-3‘ phosphodiester junction have been conveniently synthesized through a solid phase procedure involving only 3‘-phosphoramidite nucleosides, starting from a modified support linking the first nucleotide through the base. Thermal denaturation studies showed that 16-mers with this inversion of polarity cooperatively bound to 5‘-(purine)m(pyrimidine)n-3‘-type duplexes by specific recognition of the oligopurine domains alternately on the two DNA strands. The nature of the 3‘-3‘ junction and nearest neighbors affected the stability of the resulting triplexes.