We radiotracked 7 European mink Mustela lutreola Linnaeus, 1761 (5 males and 2 females), to determine their distribution, size and temporal changes of their home range in an area of southwestern Europe, where American mink M. vison was not established. Size of home ranges varied from 11 to 17 km along watercourses in males and were 0.6 and 3.6 km in females. Home ranges of males were larger than those found in previous studies. Most females captured (either radiotracked or not) were found within the home range of males. Males occupied adjoining river sections with minimal range overlap, suggesting an intrasexually exclusive spacing pattern for males. Each month males used new river stretches, mainly along tributaries; meanwhile they showed a steady use of their stem river section. Resting sites were mainly beneath dense brambles of Rubus patches located in the river bank.