This experiment was carried out during two successive seasons of 2007 / 2008 and 2008 / 2009 in Ajloun region , to study the effect of organic manure (farmyard manure and poultry manure at a rate of 75 m 3 / ha as fertilizer of each) and biofertilizers (Nitraboein and Halex-2 at a rate of 988 gm / ha of each as well) as their interactions on growth , yield of herb and volatile oil of Majorana hortensis L. , using drip irrigation system. The results indicated that the application of a poultry manure as an organic fertilizer to marjoram plants recorded the maximum values of herb fresh and dry yield , N , P and K contents and its uptake by herb in the early cut , volatile oil percentage , oil yield / plant and per hectare , while in the late cut , the highest values in this respect were obtained by farmyard manure application. Marjoram transplant treated with halex-2 biofertilizers gave the highest values of herb fresh and dry yield , N , P and K contents and its uptake by herb in the early cut , in addition to , volatile oil percentage , oil yield / plant per hectare , while plants treated with nitrobein biofertilizers gave intermediate values at different cut in this respect. The combination of poultry manure and halex-2 proved to be the superior treatments , which reflected in the greatest influence upon all the studied characters , that is , fresh and dry herb yields , mineral contents , highest volatile oil percentage and oil yield / plant of marjoram plant , followed by FYM combined with the same biofertilizers and may be recommended under similar conditions of this work .