The present study describes the distribution of RV in bovine and human population from Uttarakhand and North eastern region of India. Group A rotavirus (RVA) was detected in 17.33% and 15.68% diarrhoeic cases from bovine and humans, respectively. All bovine RVA isolates were long electropherotypes whereas, in human RVA isolates both long and short electropherotypes were detected. Genotyping by multiplex RT-PCR revealed presence of G3 and G10 genotypes in bovine and G1 and G3 in humans. Among P types, P[1] and P[11] in bovine and P[4] and P[8] in human were detected. The G and P type combinations present were G3P[11], G10P[1], G3P[1]P[11], G10P[1]P[11], G3G10P[1]P[11], G3G6G10P[1] and G3G6G10P[11] in bovine, while G1P[4], G3P[8] and G1G3P[8] in human samples.