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Nature Research, Nature Biotechnology, 4(25), p. 443-445, 2007

DOI: 10.1038/nbt1293

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Reporter proteins for in vivo fluorescence without oxygen

Journal article published in 2007 by Thomas Drepper, Thorsten Eggert, Franco Circolone, Achim Heck ORCID, Ulrich Krauss, Jan-Karl Guterl, Marion Wendorff, Aba Losi ORCID, Wolfgang Gärtner ORCID, Karl-Erich Jaeger
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Fluorescent reporter proteins such as green fluorescent protein are valuable noninvasive molecular tools for in vivo real-time imaging of living specimens. However, their use is generally restricted to aerobic systems, as the formation of their chromophores strictly requires oxygen. Starting with blue-light photoreceptors from Bacillus subtilis and Pseudomonas putida that contain light-oxygen-voltage-sensing domains, we engineered flavin mononucleotide-based fluorescent proteins that can be used as fluorescent reporters in both aerobic and anaerobic biological systems.