Indices obtained from the second derivative of the digital volume pulse waveform have been proposed to characterize vascular aging, arterial rigidity, and the effects of vasoactive drugs. The purpose of this study was to assess the effects of manual needling of PC6 on SD_DVP indices in healthy and untreated hypertensive subjects. AI, B:A, and D:A indices, based on the height of the wave components of SD_DVP, in 40 healthy subjects and 25 untreated hypertensive subjects were compared. DVP was obtained by measuring infrared light transmission through the finger. For each subject, 20-min-long DVP registration was obtained. PC6 was stimulated unilaterally by manual needling for 5 min (1–6 min). In each subject, pre-acupuncture DVP indices were compared to those of during acupuncture (1 vs. 4 min) and post-acupuncture (1 vs. 18 min). In healthy subjects, AI was significantly improved when comparing the pre- to the post-acupuncture values. In hypertensive subjects, the SD_DVP indices improved significantly as follows: the AI index when the value of pre-acupuncture comparing to that of during acupuncture and post-acupuncture values; B:A and D:A indices when the pre-comparing to post-acupuncture values. There were significant differences between healthy and hypertensive subjects in AI, B:A, and D:A at baseline and in B:A in the during acupuncture period; there were no significant differences at post-acupuncture. These results indicate that manual needling of PC6 produced acute effects on vascular pathophysiology. Moreover, PC6 needling produced changes in SD_DVP indices related to both large artery stiffness and the reflected wave originating in small arteries.