2008): Fructification of Langermannia gigantea in artificially inoculated field soil. – Czech Mycol. 60(2): 231–242. Cultures of Langermannia gigantea, a fungus potentially important for human alimentation and biotechnology purposes, were isolated from wild-growing specimens and a spawn was produced using autoclaved soil as a cultivation substrate. The spawn of two isolates was placed into the soil in two field experimental plots. Fructification was first observed after 4 years in only one plot, where 3 normal fruitbodies were produced. Analysis of the rDNA cassette of the 3 fruitbodies revealed that they were identical and bore the attributes of both introduced isolates. The fungus is able to persist in the inocu-lated soil for years and is dispersed over the soil volume. This is the first report of successful fructifica-tion of L. gigantea introduced at a locality where this species had never been observed before.