(A–C) Superimposition of the averaged injection site position in the DTh of wild-type (green) and Netrin-1−/− (red) embryos for thalamic axons crossing the CSB along its dorsal (B) or ventral (C) halves as shown in (A). (D–F) Statistical analysis using a two-way ANOVA test to determine the significance of the density maps between wild-type (WT) and Netrin-1 knockout embryos shown in (B and C). Significance was arbitrarily set at p < 0.001 with green representing bins in which the averaged density observed in WT is superior to Netrin-1−/− and vice versa for red bins. Any comparison with p > 0.001 was considered nnonsignificant and shown in white. (1.17 MB TIF)