Monocyte-γδ T cell crosstalk depends on soluble mediators. (A) MFI of CD14 and CD40 for monocytes after 18 hours of culture in medium alone or with GM-CSF+IL-4, and for monocytes separated from monocyte-γδ T cell co-cultures without or with HMB-PP and anti-IFN-γ+sTNFR (data from two individual donors). (B) Microscopic analysis of monocytes and γδ T cells co-cultured for 18 hours in the presence of HMB-PP and the blocking reagents indicated. Data shown are representative of two individual donors. (0.22 MB PDF)