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Production and partial characterization of extracellular xylanase from acidophilic Aspergillus flavus MTCC 9390 grown in SSF mode

Journal article published in 2014 by Bharat Bhushan ORCID, Ajay Pal, Satish Kumar
This paper is available in a repository.
This paper is available in a repository.

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Abstract

Multi-step screening comprising of qualitative and quantitative assays was carried out to isolate hypersecreting xylanolytic microbial strain. The enzyme super-secreter Aspergillus flavus MTCC 9390 was selected for optimizing xylanase production in solid state fermentation where various process variables like temperature, carbon source and moistening agents were optimized using conventional 'change one-variable-At-A-time' approach. A significant increase in enzyme production was achieved by change in fermenting substrate and switching it over to horticultural waste material. A further increase in xylanase production was achieved when the spent mass was agitated at warm temperature with minimal volume of extractant such as tween-80. The extracellular xylanase was fractionated with ammonium sulfate and characterized with respect to reaction parameters. The crude xylanase was active at 60°C and pH 6.0 and stability experiments revealed its better biochemical characteristics for its commercial adoption in juice industries.