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American Society for Microbiology, Journal of Clinical Microbiology, 1(51), p. 295-298, 2013

DOI: 10.1128/jcm.02221-12

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Development of a Peptide Nucleic Acid Probe to Trichosporon Species and Identification of Trichosporonosis by Use ofIn SituHybridization in Formalin-Fixed and Paraffin-Embedded (FFPE) Sections

Journal article published in 2012 by Minoru Shinozaki, Yoichiro Okubo ORCID, Daisuke Sasai, Haruo Nakayama, Somay Yamagata Murayama, Tadashi Ide, Megumi Wakayama, Takao Ishiwatari, Naobumi Tochigi, Tetsuo Nemoto, Kazutoshi Shibuya
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

In order to identify Trichosporon species in formalin-fixed and paraffin-embedded sections from which visual discrimination of non-glabrata Candida species is mostly ineffective but critical for the choice of antifungals, we tested the usefulness of a newly designed peptide nucleic acid probe (PNA) for in situ hybridization (ISH). Results confirmed the usefulness of ISH with our PNA probe in identifying Trichosporon species from Candida albicans. Copyright © 2013, American Society for Microbiology. All Rights Reserved.