Abstract: Zuckerman postulated a biopsychological multilevel theory of Sensation Seeking, which is part of a more complex multi-trait theory, the Alternative Five. The Sensation Seeking Scale Form V (SSS V) was developed for the measurement of Sensation Seeking. The process of validation of Sensation Seeking as part of a multilevel theory includes analyses of relations within and between several levels of measurement. The present study investigates validity and basic psychometric properties of a German version of the SSS V in a broader context of psychometric traits. - The 120 participants were mainly students. They completed the SSS V, the Venturesomeness- and Impulsiveness-Scales of the IVE, the BIS/BAS-Scales, the ZKPQ and the NEO-FFI. - The results reveal acceptable psychometric properties for the SSS V but with limitations with regard to factor structure. Indications for criterion validity were obtained by prediction of substance use by the subscales Dis and BS. The results of a MTMM analysis, especially the convergent validities of the SSS V were quite satisfying. On the whole, the results yielded sufficient support for the validity of the Sensation Seeking construct or the instrument respectively. They also point to desirable modifications.