Published in
American Chemical Society, Journal of Physical Chemistry B (Soft Condensed Matter and Biophysical Chemistry), 30(119), p. 9592-9600, 2015
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Analysis of Potential Binding Sites of 3,5,4′-Trihydroxystilbene (Resveratrol) and trans -3,3′,5,5′-Tetrahydroxy-4′-methoxystilbene (THMS) to the GAPDH Molecule Using a Computational Ligand-Docking Method: Structural and Functional Changes in GAPDH Induced by the Examined Polyphenols
,
Joanna Strumillo,
Eligiusz Serafin,
Mieczyslaw Puchala
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- Must obtain written permission from Editor
- Must not violate ACS ethical Guidelines
Abstract
The presented study analyzed potential binding sites of 3,5,4'-trihydroxystilbene (resveratrol, RSV) and its derivative, trans-3,3',5,5'-tetrahydroxy-4'-methoxystilbene (THMS) to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The effects of stilbene analogs on the structure of GAPDH were determined by fluorescence spectroscopy and zeta potential measurements. It was also assessed to what extent the studied compounds affect the activity of the enzyme. A computational ligand-docking study showed that there are 11 potential binding sites of RSV and 8 such sites of THMS in the GAPDH molecule. While resveratrol does not significantly affect the activity of the dehydrogenase upon binding to it, THMS leads to approx. 10% inactivation of this enzyme. THMS has no effect on GAPDH inactivation induced by the superoxide anion radical, in contrast to resveratrol, which increases dehydrogenase inactivation.