A rapid method for the liquid chromatography-tandem mass spectrometric determination of type A and B trichothecenes and their major metabolites in chicken meat, pork, chicken liver, and swine liver was developed. The analytes included T-2 toxin, HT-2 toxin, T-2 triol, neosolaniol, deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, deepoxy-deoxynivalenol and nivalenol. Compounds were extracted from samples with acetonitrile/ethyl acetate (1:3, v/v), then cleaned up using Oasis HLB cartridges. Analysis was carried out on ultra-performance liquid chromatography-tandem mass spectrometry. The mean recoveries of spiked samples ranged from 74.1-96.9% with the intra-day and inter-day relative standard deviation of less than 9.9% and 9.1%, respectively. Limit of detection and limit of quantitation ranged from 3.0-15.0 µg/kg and 10.0-50.0 µg/kg, respectively. The proposed method has been successfully applied for analysis of real samples, with primary results indicating that, compared to mycotoxins themselves, their metabolites are more likely to occur and be detectable in animal tissue foods.