Air-stable droplet interface bilayers (airDIBs) on oil-infused surfaces are versatile model membranes for synthetic biology applications, including biosensing of airborne species. However, airDIBs are subject to evaporation, which can, over time, destabilize them and reduce their useful lifetime compared to traditional DIBs that are fully submerged in oil. Here, we show that lifetimes of airDIBs can be extended by as much as an order of magnitude by maintaining the temperature just above the dew point. We find that raising the temperature from near the dew point (which was 7° C at 38.5 % relative humidity and 22° C air temperature) to 20° C results in loss of hydrated water molecules from the polar head groups of the lipid bilayer membrane due to evaporation, resulting in a phase transition with increased disorder. This dehydration transition affects primarily the bilayer electrical resistance, by increasing permeability through an increasingly disordered polar head group region of the bilayer. Temperature and/or relative humidity are conveniently tunable parameters for controlling the stability and composition of airDIBs membranes, while still allowing for operation in ambient environments.