Amino acid sequences alignment of Oxidored_FMN domain. Amino acid sequences alignment of Oxidored_FMN domain in OPR genes from eleven species representing six major lineages within the green plants: the green algae (Chlamydomonas reinhardtii and Volvox carteri), the mosses (Physcomitrella patens), the lycophytes (Selaginella moellendorffii), the gymnosperms (Picea sitchensis), the monocotyledoneous angiosperms (Oryza sativa, Sorghum bicolor and Zea mays) and the dicotyledoneous angiosperms (Arabidopsis thaliana, Populus trichocarpa and Medicago truncatula). Only 3 of 74 OPR genes (AtOPR01-2/3 and SbOPR06-4) without complete Oxidored_FMN domain are excluded, while the outgroup ScOYE1 from yeast is included in the alignment. The blue blocks below the alignment represent the fragments selected by the Gblocks program with default parameters underlined. Positions of the alignments where more than 50% of the sequences are identical are shown with black boxes. The secondary structure elements are represented at the bottom of the alignment as tubes or arrows shown in gray based on structural information from OPR proteins AtOPR1, AtOPR3 and LeOPR3. The intron positions of putative ancestral OPR are represented at the top of the alignment as red vertical arrows, and I1~I12 represent intron 1~12. The seven conserved OPR subfamilies are represented as I, II, III, IV, V, VI and VII at the left side of the alignment.