The vertical arrangement of forest canopies is a key descriptor of canopy structure, a driver of ecosystem function and indicative of forest successional stage. Yet techniques to attribute for canopy vertical structure across large and potentially heterogeneously forested areas remain elusive. This study introduces a new technique to estimate the Number of Strata (NoS) that comprise a canopy profile, using discrete-return Airborne Laser Scanning (ALS) data. Vertically resolved gap probability (P-gap) aggregated over a plot is generalized with a nonparametric cubic spline regression (P-s). Subsequently a count of the positive zero-crossings of second derivative of 1 - P-s is used to estimate NoS. Comparison with inventory derived estimates at 24 plots across three diverse study areas shows a good agreement between the two techniques (RMSE=041 strata). Furthermore, this is achieved without altering model parameters, indicating the transferability of the technique across diverse forest types. NoS values ranged from 0 to 4 at a further 239 plots, emphasizing the need for a method to quantify canopy vertical structure across forested landscapes. Comparison of NoS with other commonly derived ALS descriptors of canopy structure (canopy height, canopy cover and return height coefficient of determination) returned only a moderate correlation (r(2)