The influence of supplementing the diet of broiler breeder hens with arginine (Arg) on their offspring's humoral and cell-mediated immune response was evaluated in two experiments. In experiments I and II, breeder hens were fed diets containing graded levels of Arg (0.943, 1.093, 1.243, 1.393 and 1.543% digestible Arg). In experiment I, the offspring was randomly grouped according to the treatment received by the breeder hens, with five levels of Arg in the maternal diet and six replicates, giving a total 30 experimental units. In experiment II, the offspring were grouped in accordance with the treatment received by the breeder hens; however, Arg was added to the starter diet (1.300, 1.450, 1.600, 1.750 and 1.900% digestible Arg) and also the growing diet (1.150, 1.300, 1.450, 1.600 and 1.750% digestible Arg). Supplementation of the broiler breeder hen diet did not influence (p> 0.05) the development of the lymphoid organs (cloacal bursa, thymus and spleen) of the offspring, whether their diet were supplemented or not. Nevertheless, greater weight and dimensions cloacal bursa were found in the supplemented offspring in comparison with the non-supplemented offspring. Macrophage phagocytic activity was found to be unaffected (p> 0.05), independently of the Arg supplementation. The offspring fed with supplemented diets showed a linear reduction in the antibody titer against Newcastle Disease (p< 0.05) at seven days of age, and a quadratic response (p< 0.05) at 28 days of age. The antibody titer in the non-supplemented offspring was not influenced (p> 0.05) by the breeder hen diet. This study concluded that supplementing the breeder hen diet with arginine is insufficient to improve the humoral and cellular immune response, requiring supplementation of the offspring diet.