Canopy stratification is one of the oldest concepts in tropical forest ecology. However, there has been considerable debate over the existence and identification of strata. Much of the confusion arises from the differing definitions of strata (i.e. vertical stratification of phytomass, individual crowns, or species) and the methods used to evaluate them (e.g. profile diagrams). In this paper, a quantitative technique for identifying stratification of individual tree crowns in the forest canopy is presented. Strata are identified by comparing sorted tree heights to a moving average of height at the base of the live crown. Height and crown measurements were obtained from 21 published profile diagrams of forests, representing many biogeographic regions and covering a wide variety of forest types. The technique provides an objective measure of canopy strata allowing for a valid comparison of stratification between the different profile diagrams. Neither the original author's estimates of strata nor the number of strata detected by the quantitative technique support the premise that tropical forests have more strata than temperate forests. With the sole exception of a mono-layered European Douglas-fir plantation, all forests in this study had two or three layers.