Occurrence of Eurasian lynx (Lynx lynx, Linnaeus 1758) was studied in the broader area of the Beskydy Protected Landscape Area (PLA) in the West Carpathians. The monitoring of the species was based on detection of indirect signs of presence (tracks, scat, remains of prey) found during the monitoring events conducted by trained volunteers of Friends of the Earth Czech Republic. Confirmed signs of lynx presence ("C2" according to the SCALP methodology) were analysed together with all walked trails in KFME subsquares and relative lynx abundance was calculated. Camera traps were additionally used during 2009–2012 for confirmation of species presence and its possible reproduction. Eurasian lynx presence was confirmed throughout the study area but it differed between the geomorphological units and it changed during the study period. There was significantly lower lynx abundance in the Moravskoslezské Beskydy Mts. and Vsetínské vrchy Hills comparing to the Javorníky Mts. The index of lynx numbers decreased in the Vsetínské vrchy Hills, increased in the Javorníky Mts. and became stable in the Moravskoslezské Beskydy Mts. There were 15 lynx kittens older than 6 months detected in the Javorníky Mts. and 5 in the Moravskoslezské Beskydy Mts., what did a significant difference. Average litter size was 2,22 ± 0,86. Fluctuation of lynx numbers and lower abundance in some areas coincided with non-confirmed cases of poaching, reported by local people from the region. Apart from the Beskydy PLA, lynx presence was confirmed also in the Vizovické vrchy Hills and in the northern part of the Bílé Karpaty Mts.