In the summer of 1992 the pre-pilot phase of the NCI/EPA Agricultural Health Study (AHS) was initiated to evaluate environmental sampling and analysis methods for assessing the exposure of farm workers and their families to selected pesticides used in and around the farm. ampling media consisted of polyurethane form (PUF) for ambient and breathing zone air monitoring; gloves, a-cellulose patches and isopropanol handwipes for personal exposure monitoring; and soil and housedust samples for environmental monitoring. n initial target compound list of 26 herbicides, insecticides and fungicides was selected. he target compound list was supplemented, for selected samples, with two insecticides and two herbicides following field sampling. he pesticides were separated into three classes: organochlorine pesticides, organophosphates and carbamates, and acids. he wide variety of matrices and pesticides presented a challenge since no single extraction method or analytical method could be used for all matrices or for all target analytes. his presentation will describe the extraction and analysis methodology used for this project. n addition, method validation results, laboratory spike results and blind external audit sample results are presented.