Chloramphenicol (CAP) residues were determined in 35 samples of royal jelly. Fourteen sam- ples were collected by Veterinary Border Inspection officials in Milan and Turin and 21 were submitted by private importers to confirm the quality of their product. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used for the identification and determination of CAP, according to the isotope dilution approach for the analysis of royal jelly. The presence of chloramphenicol was detected in 29 out of 35 tested samples at concentrations ranging from 0.6 µg/kg to 28 µg/kg, with an average content of 6.1 µg/kg. royal jelly / chloramphenicol / residue / antibiotics / liquid chromatography-tandem mass spectrom- etry