In this paper, we report evidences that cellular distribution of phosphofructokinase can be affected by epinephrine stimulation in rabbit skeletal muscle homogenates. Through co-sedimentation techniques, we observed that in epinephrine-stimulated tissues, approximately 50% of phosphofructokinase activity is co-located in an actin-enriched fraction, against 29% in control. This phenomenon is accompanied by a 400% increase in specific phosphofructokinase activity in stimulated homogenates. This effect is reproduced by the beta-adrenergic agonist isoprenaline. Here we propose that the modulation of cellular distribution of phosphofructokinase may be one of the mechanisms of control of glycolytic flux in mammalian muscle, by beta-adrenergic stimulation.