The study aims to estimate the relative contributions of the two drainage basins of the Aliakmon and Axios rivers which, since the Mid-Holocene, have been responsible for building the largest deltaic area in Greece. Sediments from five cores located in the central part of the Thessaloniki Plain have been studied for their environmental changes using paleontological and sedimentological methods. Chronostratigraphical evidence was obtained from 14C AMS dating of marine shells, peat and organic sediment samples. During the Holocene marine transgression, this large coastal plain was a shallow marine bay reaching approx. 35 km inland circa the 4th millennium BC, from which the sea subsequently regressed to the east. Around the middle of the 3rd millennium BC, strong fluvial deposition of Aliakmon, to the east, and of Axios, to the north, occurred and was responsible of a gradual change to lagoonal and limnic environmental conditions. Around the 5th Century BC, a freshwater lake occupied the westernmost part of the plain.Microfaunal identification, together with magnetic susceptibility measurements, and grain-size analysis reveal three main environments of sediment deposition that reflect combinations of both concentrated and dispersed sources of magnetic/source minerals. Using remote sensing and a combination of spectral bands (LANDSAT TM imagery), we identify former fluvial levees and a freshwater lake, and give a spatial interpretation of the rivers' influences in building this deltaic complex. The mechanisms of edification of the plain as well as the roles played by Aliakmon and Axios sedimentation are described.