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Nature Research, Nature Biotechnology, 12(27), p. 1173-1175, 2009

DOI: 10.1038/nbt.1589

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High-resolution analysis of DNA regulatory elements by synthetic saturation mutagenesis

Journal article published in 2009 by Rupali P. Patwardhan, Choli Lee, Oren Litvin, David L. Young, Dana Pe'er, Jay Shendure ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

We present a method that harnesses massively parallel DNA synthesis and sequencing for the high-throughput functional analysis of regulatory sequences at single-nucleotide resolution. As a proof of concept, we quantitatively assayed the effects of all possible single-nucleotide mutations for three bacteriophage promoters and three mammalian core promoters in a single experiment per promoter. The method may also serve as a rapid screening tool for regulatory element engineering in synthetic biology.