The influence of riverine inputs on biogeochemical cycling and organic matter recycling in sediments on the continental shelf off the Rhône River mouth (NW Mediterranean Sea) was investigated by measuring sediment oxygen uptake rates using a combination of in situ and laboratory techniques. Four stations were investigated during two cruises in June 2001 and June 2002, with depths ranging from 9 to 192 m and over a distance to the Rhône River mouth ranging from 4 to 36 km. Diffusive oxygen uptake (DOU) rates were determined using an in situ sediment microprofiler and total oxygen uptake (TOU) rates were measured using sediment core incubations. There was good agreement between these two techniques which indicates that the non-diffusive fraction of the oxygen flux was minimal at the investigated stations. DOU rates ranged from 3.7±0.4 mmol O2 m−2 d−1 at the continental shelf break to 19.3±0.5 mmol O2 m−2 d−1 in front of the Rhône River mouth. Sediment oxygen uptake rates mostly decreased with increasing depth and with distance from the Rhône mouth. The highest oxygen uptake rate was observed at 63 m on the Rhône prodelta, corresponding to intense remineralization of organic matter. This oxygen uptake rate was much larger than expected for the increasing bathymetry, which indicates that biogeochemical cycles and benthic deposition are largely influenced by the Rhône River inputs. This functioning was also supported by the detailed spatial distribution of total organic carbon (TOC), total nitrogen (TN) and C/N atomic ratio in surficial sediments. Sediments of the Rhône prodelta are enriched in organic carbon (2–2.2%) relative to the continental shelf sediments (<1%) and showed C/N ratios exceeding Redfield stoichiometry for fresh marine organic matter. A positive exponential correlation was found between DOU and TOC contents (r2=0.98, n=4). South-westward of the Rhône River mouth, sediments contained highly degraded organic matter of both terrestrial and marine origin, due to direct inputs from the Rhône River, sedimentation of marine organic matter and organic material redeposition after resuspension events.