During an investigation of the stem bark and leaves of Peschiera buchtieni, 34 alkaloids were isolated. Eight new alkaloids were obtained from the stem bark, N-methyl-pericyclivine, 18,19(R)-dihydroxycoronaridine, chloromethylene affinisinium, demethylaccedinisine, 18-hydroxyaffinisine, buchtienine, 3-hydroxytetrahydroolivacine and demethylceridimine. The known alkaloids from the stem bark were coronaridine, voaphylline, coronaridine hydroxyindolenine, heyneanine, eglandine, eglandulosine, 19-epi-heyneanine, voachalotine, ochropamine, olivacine, ibogamine, affinisine, N-oxyaffinisine, vallesamine, (E)-isositsirikine, voaphylline hydroxyindolenine, normacusine B, janetine, 3,14-dihydroolivacine, 3′(R/S)-hydroxy-N-demethyl-tabernamine, 4′,17β-dihydrotchibangensine, ceridimine and N-demethyltabernamine. Apodine, voacristine, voacristine hydroxyindolenine and olivacine were identified in leaves.