The present study aimed to investigate the chemical composition of essential oil (EO) and the antioxidant and antimicrobial activities of methanol extract (Me-OH), ethyl acetate extract (ETOAC) and EO of B. carteri resin. Using gas chromatography/mass spectrometry (GC/MS), 40 components were identified in B. carteri resin essential oils, and the most abundant components were, verticiol (14.48 %), isobutylcyclopentane (12.25 %) and n-octyl acetate (9.20 %). The Me-OH extract exhibited the highest antioxidant activity using DPPH radical scavenging, Fe 2+ chelating and the reducing power assays compared to ETOAC extract and EO. Also, the Me-OH extract showed the highest antimicrobial activity against all the tested bacterial and yeast microorganisms compared to ETOAC extract and EO. The Me-OH and ETOAC extracts exhibited the highest antimicrobial activity against Streptococcus faecalis, Bacillus subtilis and Bacillus circulans with MIC value 25μg/ml. In conclusion, Me-OH and ETOAC extracts as well as EO of B. carteri resin may be considered as a natural source for antimicrobial and antioxidant agents and could be used for application in pharmaceutical and food industries.