Development of CRABP2 knock down clones. (A) Scheme of the short hairpin (sh) RNA sequences cloned into the pSUPER vector and subsequently used for HME1 stable transfection (left). Transient co-transfection experiments followed by WB analysis showing that the CRABP2-targeting sequences CRABP2-A and CRABP2-C, but not the scrambled sequence Mock, can effectively decrease the protein level of exogenous CRABP2 (right). (C) Sequencing analyses showing that the stable clones Si-CRABP2-A6, Si-CRABP2-C6 and Mock-13 contain the correct p-SUPER construct. (0.76 MB DOC)