The present investigation was aim to study the antimicrobial potential of methanol extract and zinc oxide nanoparticles synthesized from Emblica officinalis (E. officinalis). The antimicrobial activity was performed by agar well diffusion method against six bacterial pathogens namely, Bacillus subtilis, Streptococcus pneumonia, Staphylococcus epidermidis, Klebsiella pneumonia, Salmonella typhi and Escherichia coli, and two fungal pathogens such as, Aspergillus niger and candida albicans. The minimal inhibitory concentration (MIC) was evaluated by micro-broth dilution method against selected pathogens and also minimum bactericidal concentration (MBC) reported. The results showed both methanol extract and zinc oxide nanoparticles harbor significant antimicrobial activity on most of the tested organisms. Highest antimicrobial activity was observed in zinc oxide nanoparticles against S. epidermidis (35 mm) with minimal MIC and MBC values (7.81 μg/ml) followed by E. coli. Both methanol extract and zinc oxide nanop rticles were showed nil activity on fungal pathogens. The present results concluded that green synthesized zinc oxide nanoparticles from E. officinalis may be severed as an effective antibacterial agent in traditional medicine.