Competitive effect of anti-HLA class I monoclonal antibody (01.65) and N-N-staurosporine on proliferative response of PHA activated T-lymphocytes
Journal article published in 1991 by
R. Selvatici 
,
P. Orlando,
M. Rubini ,
A. Balboni,
S. Balugani,
E. Gandini
,
P. Orlando,
M. Rubini ,
A. Balboni,
S. Balugani,
E. Gandini
Abstract
N-N-Staurosporine (STAR) inhibits in a dose dependent manner Tritiated-Thymidine (3H-TdR) incorporation in phytohemagglutinin (PHA) activated Peripheral Blood Mononuclear Cells (PBMC) treated with anti-HLA class I monoclonal antibody (MAb) 01.65 and its effect results competitive with MAb 01.65. Cytosolic and particulate Protein Kinase C (PKC) have been studied. Only when PKC particulate activity is no more detectable, the effect of STAR on 3H-TdR incorporation is evident.