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Springer, Chromatographia, 3(25), p. 248-248, 1988

DOI: 10.1007/bf02316459

Springer, Chromatographia, 11(23), p. 796-802, 1987

DOI: 10.1007/bf02311402

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An efficient procedure for extraction and determination of steroids in the tissue of laboratory animals

Journal article published in 1987 by A. Laganà ORCID, A. Lagana', L. Curini, R. Curini, G. d'Ascenzo, G. D’Ascenzo, A. Marino
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

A high-performance liquid chromatographic method for analysis of steroids in the tissue of laboratory animals is described. A two-stage solid extraction procedure, utilizing Sep-pak C18 and graphitized carbon black, gives a purified product. Steroids in this extract were separated by multistep gradient elution with a reversedphase liquid chromatography system, using acetonitrilewater as solvent. Several columns with different geometry and phase loading were investigated. Optimum resolution was obtained with a medium covered and spherical particle.The applicability of this method was successfully demonstrated in the analysis of the testis and adrenal gland of rat.