Surface modification of alumina nanomembrane (ANP) by theophylline derivative Theo1 by dip-coating was successfully achieved. The hybrid material was characterized by SEM, XPS and confocal microscopy. The presence of Theo1 on the ANP surface was established from salt diffusion and membrane potentials measurements. Changes in the electrical character and transport parameters of the modified sample, associated to Theo1 deposition, were established. Stability of the material under diffusive flow was also ascertained. Moreover, as a result of Theo1 bioactivation, streptavidin linkage to the ANP/Theo1-modified membrane was obtained, which is an indication of streptavidin capture.