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Taylor and Francis Group, Bioscience, Biotechnology and Biochemistry, 2(75), p. 379-381, 2011

DOI: 10.1271/bbb.100730

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A Calcium-Dependent Xylan-Binding Domain of Alkaline Xylanase from AlkaliphilicBacillussp. Strain 41M-1

Journal article published in 2011 by Risa Yazawa, Jun Takakura, Tomoko Sakata, Ihsanawati, Rie Yatsunami, Toshiaki Fukui ORCID, Takashi Kumasaka ORCID, Nobuo Tanaka, Satoshi Nakamura
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Xylanase J of alkaliphilic Bacillus sp. strain 41M-1 contains a carbohydrate-binding module family 36 xylan-binding domain (XBD). Mutational analysis of the XBD revealed that Tyr237, Asp313, Trp317, and Asp318 were involved in Ca(2+)-dependent xylan-binding, and that Asp313 and Asp318 were especially important.