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Taylor and Francis Group, Analytical Letters, 8(37), p. 1559-1570

DOI: 10.1081/al-120037587

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Thermal Inkjet Technology for the Microdeposition of Biological Molecules as a Viable Route for the Realization of Biosensors

This paper is available in a repository.
This paper is available in a repository.

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Abstract

Recent progress in inkjet printing of parts of biosensors are highlighted, with particular reference to the printing of biologically active molecules. We describe a system constituted by a thermal inkjet printer, adapted to layering a bidimensional array of dots [701 × 701 dots per inch] on solid supports. The printer was used to depose a β‐galactosidase (GAL)‐containing ink on a polyester sheet, with dots obtained from 10 pL drops, each drop containing in turn 6 pg of enzyme. The activity of GAL after the preparation was determined using a colorimetric probe (Brilliant Blue FCF). The activity loss of the microdeposed enzymes was found to be around 15%, showing that the 2 µsec‐lasting thermal shock experienced by the biomolecule into the printhead nozzle affects to a lesser extent the activity of the thermal inkjet deposited enzyme. In conclusion, the most recent findings of our group in this line are depicted, and a view of possible future developments of the “biopolytronics” field is outlined.