Effect of Notch on the activity of Armadillo in clones of cells of different ages. Confocal images of third instar wings discs with MARCM clones of Notch mutant cells (labelled in green) that overexpress ArmS10 without (A and B) and with (C and D) CeN, induced at 72–96 h (A and C), 48–72 h (B and D). (A–D) are apical, and A1–D1 are basal sections. As in Figure S2, the red channel shows Scribble (a basolateral cell junction marker) and as in other figures, the pictures on the top and the right represent optical z-section through the clones following the green and the red lines shown in the main picture. The small insets at the corners of each image are low magnification pictures of the discs shown, which act as a reference. Note that the size and appearance of the clones change depending on the stage of the induction: the late induced clones are smaller and exhibit an irregular shape (A), while the early ones are bigger and rounder (B). Often the clones lose the basal connexion (white arrow in [A]), some can be seen to coalesce (with one in the peripodial membrane in [B], white arrowheads), and some cells within the clones lose their polarity (blue arrows in [B]). The clones in the wing disc cells are depicted with a continuous line, and those in the peripodial membrane in a dashed line. Expression of CeN rescues the effect of the loss of function of Notch on the activity of ArmS10 (C and D). These clones are smaller, they do not fuse, recover their polarity and span the epithelium. The complete z-stack of (B) and (D) can be found as Videos S2 and S3, respectively. Scale bar, 20 µm. (6.72 MB TIF)