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Oxford University Press, FEMS Microbiology Letters, 1(142), p. 111-116, 1996

DOI: 10.1111/j.1574-6968.1996.tb08416.x

Oxford University Press (OUP), FEMS Microbiology Letters, 1(142), p. 111-116

DOI: 10.1016/0378-1097(96)00252-2

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Cloning of thefliIgene fromRhodobacter sphaeroidesWS8 by analysis of a transposon mutant with impaired motility

Journal article published in 1996 by Ian Gordon Goodfellow ORCID, Charles Edward Pollitt, Renee Elizabeth Sockett
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

A transposon mutant of Rhodobacter sphaeroides WS8 was isolated that showed reduced swarming on soft agar plates. Liquid cultures of this mutant (M18) showed a low percentage of motile swimming cells in mid-exponential phase and a low level of extracellular flagellin protein by Western blotting. M18 was complemented by a clone from a library of R. sphaeroides WS8 DNA, and restriction mapping of the site of TnphoA insertion in the mutant, coupled with DNA sequencing, showed that it had a defect in the fliI gene. To determine if a partly functional fliI gene was giving the low-motility phenotype of M18, a drug resistance omega cartridge was inserted into the gene to give a complete null mutant. This null strain also produced a low percentage of motile cells. Possible reasons for this apparent fliI-independent flagellar formation are discussed.